Hotspot

trnS-GCU-trnG-UCC

LSC · 9048-9819 · 772 bp

Back to Caiophora

Pi 0.00649
MarkerSeek score 51.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
772
variable_sites
5
pi
0.00649
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA CGGAATAGGAATCCAAACCGA 50.0 47.6 58.0 57.8 894 bp 1.0 71.0
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA TCGGAATAGGAATCCAAACCG 50.0 47.6 58.0 57.8 895 bp 1.0 71.0
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC CGGAATAGGAATCCAAACCGA 50.0 47.6 58.0 57.8 893 bp 1.0 71.0
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TCGGAATAGGAATCCAAACCG 50.0 47.6 58.0 57.8 894 bp 1.0 71.0
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TCCAAACCGAAAGATCCCTT 50.0 45.0 58.0 56.7 883 bp 1.0 70.4