Hotspot

ycf1

SSC · 125692-131079 · 5388 bp

Back to Cadaba

Pi 0.0113
MarkerSeek score 44.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5388
variable_sites
61
pi
0.0113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CAGTTAGTGAAGAATTTGTCCA AGTCGTACAAGAAATTAAAAACTGGA 36.4 30.8 54.3 57.6 218 bp 1.0 42.2
ycf1_p2 CAGTTAGTGAAGAATTTGTCCA GTCGTACAAGAAATTAAAAACTGGA 36.4 32.0 54.3 56.3 217 bp 1.0 42.2
ycf1_p3 CAGTTAGTGAAGAATTTGTCCA AGTCGTACAAGAAATTAAAAACTGG 36.4 32.0 54.3 56.3 218 bp 1.0 42.2
ycf1_p4 CAGTTAGTGAAGAATTTGTCCA GTCGTACAAGAAATTAAAAACTGG 36.4 33.3 54.3 55.0 217 bp 1.0 42.2
ycf1_p5 CAGTTAGTGAAGAATTTGTCCA AGAGCAAAAATTTTTCTAAAAGTCGT 36.4 26.9 54.3 57.1 238 bp 1.0 42.0