Hotspot

ndhF

SSC · 111958-114204 · 2247 bp

Pi 0.0107

MarkerSeek score 55.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 24
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATGATGAATTCCACGTTCG	AGAAACCAGTGAATCAGAAA	47.6	35.0	57.4	52.3	2384 bp	1.0	40.8
ndhF_p2	GGATGATGAATTCCACGTTCG	AGATAGAAACCAGTGAATCAGA	47.6	36.4	57.4	54.3	2388 bp	1.0	40.8
ndhF_p3	GGATGATGAATTCCACGTTCG	AAGATAGAAACCAGTGAATCAGA	47.6	34.8	57.4	55.1	2389 bp	1.0	40.8
ndhF_p4	GGATGATGAATTCCACGTTCG	AGATAGAAACCAGTGAATCAGAA	47.6	34.8	57.4	55.1	2388 bp	1.0	40.8
ndhF_p5	GGATGATGAATTCCACGTTCG	AGAAACCAGTGAATCAGAAAT	47.6	33.3	57.4	53.0	2384 bp	1.0	40.8