Hotspot

infA

LSC · 81070-81177 · 108 bp

Back to Cadaba

Pi 0.037
MarkerSeek score 63.90
Primer available yes
Label infA

Feature Metadata

label_name
infA
feature_type
gene
region
LSC
length_bp
108
variable_sites
4
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA_p1 ATGTCCCCTGGTTAACTCAT CTGAATCACTTCCGAATGGC 45.0 50.0 55.8 56.9 170-176 bp 1.0 55.2
infA_p2 GTCCCCTGGTTAACTCATACA CTGAATCACTTCCGAATGGC 47.6 50.0 56.7 56.9 168-174 bp 1.0 54.7
infA_p3 TGTCCCCTGGTTAACTCATAC CTGAATCACTTCCGAATGGC 47.6 50.0 56.7 56.9 169-175 bp 1.0 54.7
infA_p4 ATGTCCCCTGGTTAACTCAT TCTGAATCACTTCCGAATGGC 45.0 47.6 55.8 58.4 171-177 bp 1.0 53.2
infA_p5 TGTCCCCTGGTTAACTCATACA CTGAATCACTTCCGAATGGC 45.5 50.0 58.5 56.9 169-175 bp 1.0 53.1