Hotspot

ndhF-rpl32

SSC · 114205-115172 · 968 bp

Back to Cadaba

Pi 0.0374
MarkerSeek score 72.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
968
variable_sites
35
pi
0.0374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTGGAATGAAAGGTATGACCCA AAAAGCTTTCAATGCTGCCC 45.5 45.0 58.0 57.8 1096-1110 bp 1.0 67.2
ndhF-rpl32_p2 AGTGGAATGAAAGGTATGACCC AAAAGCTTTCAATGCTGCCC 45.5 45.0 57.8 57.8 1097-1111 bp 1.0 65.6
ndhF-rpl32_p3 TGGAATGAAAGGTATGACCCA AAAAGCTTTCAATGCTGCCC 42.9 45.0 56.6 57.8 1095-1109 bp 1.0 64.3
ndhF-rpl32_p4 GTGGAATGAAAGGTATGACCCA AGCTTTCAATGCTGCCCAAT 45.5 45.0 58.0 58.7 1093-1107 bp 1.0 62.8
ndhF-rpl32_p5 GTGGAATGAAAGGTATGACCC AAAAGCTTTCAATGCTGCCC 47.6 45.0 56.3 57.8 1096-1110 bp 1.0 61.8