Hotspot

psaJ-rpl33

LSC · 68207-68648 · 442 bp

Back to Cadaba

Pi 0.0252
MarkerSeek score 57.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
442
variable_sites
11
pi
0.0252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTAGGGGTTGGGAGTTACGT TCGTTTCGAACACAACTGGT 50.0 45.0 58.0 58.0 754-758 bp 1.0 81.7
psaJ-rpl33_p2 ACCCGTGACATTTTGTACCC TCGTTTCGAACACAACTGGT 50.0 45.0 58.1 58.0 1059-1060 bp 1.0 80.7
psaJ-rpl33_p3 CCCGTGACATTTTGTACCCA TCGTTTCGAACACAACTGGT 50.0 45.0 58.1 58.0 1058-1059 bp 1.0 80.7
psaJ-rpl33_p4 ACAGGATTTGAACCCGTGAC TCGTTTCGAACACAACTGGT 50.0 45.0 58.1 58.0 1070-1071 bp 1.0 80.6
psaJ-rpl33_p5 CAGGATTTGAACCCGTGACA TCGTTTCGAACACAACTGGT 50.0 45.0 58.1 58.0 1069-1070 bp 1.0 80.6