Hotspot

psaA-ycf3

LSC · 42841-43506 · 666 bp

Back to Cadaba

Pi 0.0105
MarkerSeek score 63.50
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
666
variable_sites
7
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 777-786 bp 1.0 78.4
psaA-ycf3_p2 AGAAATGACCGGGTTTAGCC TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 794-803 bp 1.0 78.3
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 840-849 bp 1.0 77.7
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT CAAGCCGCTGAGTATTGGAA 50.0 50.0 57.9 58.3 842-851 bp 1.0 77.7
psaA-ycf3_p5 AGAAATGACCGGGTTTAGCC AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 857-866 bp 1.0 77.7