Hotspot

rps14-psaB

LSC · 38207-38357 · 151 bp

Back to Cadaba

Pi 0.0214
MarkerSeek score 68.80
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
151
variable_sites
3
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 TGAGGATCGACGAATCAAATGA TCTACATCGGGCAAATTCGG 40.9 50.0 57.6 58.1 245-256 bp 1.0 65.0
rps14-psaB_p2 TGAGGATCGACGAATCAAATGA ACATCGGGCAAATTCGGTTA 40.9 45.0 57.6 57.9 242-253 bp 1.0 64.5
rps14-psaB_p3 TGAGGATCGACGAATCAAATGA TACATCGGGCAAATTCGGTT 40.9 45.0 57.6 57.9 243-254 bp 1.0 64.5
rps14-psaB_p4 TGAGGATCGACGAATCAAATGA GCAGCTTTCTTGATTGCCTC 40.9 50.0 57.6 57.7 263-274 bp 1.0 62.9
rps14-psaB_p5 TGAGGATCGACGAATCAAATGA GCCTCTACATCGGGCAAATT 40.9 50.0 57.6 58.3 248-259 bp 1.0 62.8