Hotspot

rpoB-trnC-GCA

LSC · 27106-28224 · 1119 bp

Back to Cadaba

Pi 0.0136
MarkerSeek score 60.10
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1119
variable_sites
15
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTTCCCTCATTTCCATCCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1199-1207 bp 1.0 80.6
rpoB-trnC-GCA_p2 CCCTCATTTCCATCCCCAAG CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1196-1204 bp 1.0 80.6
rpoB-trnC-GCA_p3 GTTCCCTCATTTCCATCCCC GGAGTCTTTTGTTGAGGCGA 55.0 50.0 57.9 58.1 1222-1230 bp 1.0 80.3
rpoB-trnC-GCA_p4 CCCTCATTTCCATCCCCAAG GGAGTCTTTTGTTGAGGCGA 55.0 50.0 57.9 58.1 1219-1227 bp 1.0 80.2
rpoB-trnC-GCA_p5 CTCATTTCCATCCCCAAGCA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1194-1202 bp 1.0 80.0