Hotspot

trnR-UCU-atpA

LSC · 9958-10196 · 239 bp

Back to Cadaba

Pi 0.0441
MarkerSeek score 69.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
239
variable_sites
10
pi
0.0441
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC ACGTTTTCTACTTCAGGAACA 47.6 38.1 57.3 55.1 289-297 bp 1.0 46.0
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC GAACGTTTTCTACTTCAGGAACA 47.6 39.1 57.3 57.0 291-299 bp 1.0 46.0
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AACGTTTTCTACTTCAGGAACA 47.6 36.4 57.3 55.9 290-298 bp 1.0 46.0
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC ACGTTTTCTACTTCAGGAACAA 47.6 36.4 57.3 55.9 289-297 bp 1.0 46.0
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT ACGTTTTCTACTTCAGGAACA 47.6 38.1 56.9 55.1 324-332 bp 1.0 45.4