Hotspot

trnH-GUG-psbA

LSC · 82-489 · 408 bp

Back to Cadaba

Pi 0.038
MarkerSeek score 79.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
408
variable_sites
15
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 472-478 bp 1.0 83.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 486-492 bp 1.0 82.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 485-491 bp 1.0 82.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 487-493 bp 1.0 82.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TGAGGCTCCATCCACAAATG 55.0 50.0 58.1 57.9 449-455 bp 1.0 82.9