Hotspot

psaC-ndhE

SSC · 122063-122509 · 447 bp

Back to Cabomba

Pi 0.0641

MarkerSeek score 80.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 447
variable_sites: 34
pi: 0.0641
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTCGTACGCATTGGGTAC	TCTTCGTTATGGCGATTGCA	57.9	45.0	58.7	58.0	503-683 bp	1.0	74.7
psaC-ndhE_p2	GCTCGTACGCATTGGGTAC	CTTCGTTATGGCGATTGCAG	57.9	50.0	58.7	57.6	502-682 bp	1.0	71.9
psaC-ndhE_p3	GCTCGTACGCATTGGGTAC	CCGAAGCAGCTATTGGACTA	57.9	50.0	58.7	57.1	479-659 bp	1.0	67.9
psaC-ndhE_p4	GCTCGTACGCATTGGGTAC	TCGATCTTCGTTATGGCGAT	57.9	45.0	58.7	56.9	507-687 bp	1.0	66.2
psaC-ndhE_p5	GCTCGTACGCATTGGGTA	TCTTCGTTATGGCGATTGCA	55.6	45.0	57.2	58.0	503-683 bp	1.0	65.8