Hotspot

rpl32-trnL-TAG

SSC · 118313-118689 · 377 bp

Back to Cabomba

Pi 0.0674

MarkerSeek score 79.50

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 377
variable_sites: 32
pi: 0.0674
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	GGCAGCGGTAAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	487-492 bp	1.0	88.3
rpl32-trnL-TAG_p2	GGCAGCGGTAAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	488-493 bp	1.0	88.3
rpl32-trnL-TAG_p3	TGCCATTGCGTCAATCCTAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	961 bp	0.333333	52.3
rpl32-trnL-TAG_p4	TGCCATTGCGTCAATCCTAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	962 bp	0.333333	52.3
rpl32-trnL-TAG_p5	TGCCATTGCGTCAATCCTAA	AGGTCATCAGATGCGTAGGA	45.0	50.0	57.9	57.9	1204 bp	0.333333	51.8