Hotspot

rpl16-rps3

LSC · 87901-88063 · 163 bp

Back to Cabomba

Pi 0.0695
MarkerSeek score 80.40
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
163
variable_sites
17
pi
0.0695
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AGTACCAACGAGTCACACAC TTTGGATATTTTTAGATGAAGAGT 50.0 25.0 57.5 52.1 208-216 bp 1.0 49.4
rpl16-rps3_p2 GTACCAACGAGTCACACACT TTTGGATATTTTTAGATGAAGAGT 50.0 25.0 57.5 52.1 207-215 bp 1.0 49.4
rpl16-rps3_p3 GGAGTACCAACGAGTCACAC TTTGGATATTTTTAGATGAAGAGT 55.0 25.0 57.9 52.1 210-218 bp 1.0 49.3
rpl16-rps3_p4 GAGTACCAACGAGTCACACA TTTGGATATTTTTAGATGAAGAGT 50.0 25.0 57.2 52.1 209-217 bp 1.0 49.3
rpl16-rps3_p5 AGGAGTACCAACGAGTCACA TTTGGATATTTTTAGATGAAGAGT 50.0 25.0 58.0 52.1 211-219 bp 1.0 49.2