Hotspot

petD-rpoA

LSC · 83012-83423 · 412 bp

Back to Cabomba

Pi 0.0977

MarkerSeek score 77.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 46
pi: 0.0977
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCAGTAGCCCTTTGGTTAGG	ACAATCGACTTGCTTCAAAA	55.0	35.0	58.2	54.0	419-520 bp	1.0	58.2
petD-rpoA_p2	GCAGTAGCCCTTTGGTTAGG	ACAATCGACTTGCTTCAAAAT	55.0	33.3	58.2	54.7	419-520 bp	1.0	55.2
petD-rpoA_p3	CCCGGTAGCTACAACAGTCT	ACAATCGACTTGCTTCAAAA	55.0	35.0	58.8	54.0	453-554 bp	1.0	53.1
petD-rpoA_p4	CAGTAGCCCTTTGGTTAGGT	ACAATCGACTTGCTTCAAAA	50.0	35.0	56.8	54.0	418-519 bp	1.0	51.8
petD-rpoA_p5	CCGGTAGCTACAACAGTCTT	ACAATCGACTTGCTTCAAAA	50.0	35.0	57.0	54.0	452-553 bp	1.0	51.4