Hotspot

psbE-petL

LSC · 70047-71312 · 1266 bp

Back to Cabomba

Pi 0.0241

MarkerSeek score 71.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 43
pi: 0.0241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TTCTCCCGTACTTCCAGACA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.7	57.1	1375-1431 bp	1.0	75.8
psbE-petL_p2	TTCTCCCGTACTTCCAGACA	CCAATGAATAGAGCTGGGGT	50.0	50.0	57.7	57.0	1358-1414 bp	1.0	75.1
psbE-petL_p3	TTCTCCCGTACTTCCAGACA	ACCAATGAATAGAGCTGGGG	50.0	50.0	57.7	57.0	1359-1415 bp	1.0	75.0
psbE-petL_p4	ATATCAGCAAAAGCGCGTTC	GTCGTATCTTGCTCAGACCA	45.0	50.0	57.5	57.1	1392-1448 bp	1.0	74.4
psbE-petL_p5	TTCTCCCGTACTTCCAGACA	GACCAATGAATAGAGCTGGGG	50.0	52.4	57.7	58.1	1360-1416 bp	1.0	74.2