Hotspot

accD-trnH-ATG

LSC · 63101-63795 · 695 bp

Back to Cabomba

Pi 0.0284

MarkerSeek score 63.60

Primer available yes

Label accD-trnH-ATG

Feature Metadata

label_name: accD-trnH-ATG
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 25
pi: 0.0284
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-trnH-ATG_p1	TACATTGCATTCGCGGGTAA	CTATTTCCGCTTGTGTCCGA	45.0	50.0	58.0	58.0	1097-1363 bp	1.0	87.0
accD-trnH-ATG_p2	TACATTGCATTCGCGGGTAA	TCTATTTCCGCTTGTGTCCG	45.0	50.0	58.0	58.0	1098-1364 bp	1.0	87.0
accD-trnH-ATG_p3	TACATCCCCTACTACTGGCG	CTATTTCCGCTTGTGTCCGA	55.0	50.0	58.0	58.0	1173-1439 bp	1.0	86.9
accD-trnH-ATG_p4	TACATCCCCTACTACTGGCG	TCTATTTCCGCTTGTGTCCG	55.0	50.0	58.0	58.0	1174-1440 bp	1.0	86.9
accD-trnH-ATG_p5	TACATCCCCTACTACTGGCG	CGGTGCCTCGGTGTATTTAA	55.0	50.0	58.0	58.0	940-1206 bp	1.0	86.9