Hotspot

ndhC-trnV-TAC

LSC · 53614-55561 · 1948 bp

Back to Cabomba

Pi 0.0274

MarkerSeek score 72.80

Primer available yes

Label ndhC-trnV-TAC

Feature Metadata

label_name: ndhC-trnV-TAC
feature_type: igs
region: LSC
length_bp: 1948
variable_sites: 76
pi: 0.0274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-TAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2079-2095 bp	1.0	83.9
ndhC-trnV-TAC_p2	AATTGGGGCTAAAATCCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	2083-2099 bp	1.0	82.8
ndhC-trnV-TAC_p3	GGTCCTTCACTAATTGGGGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	2094-2110 bp	1.0	82.6
ndhC-trnV-TAC_p4	GGGGCTAAAATCCCGGAAAT	AGCCCAATGCACAAAAATGC	50.0	45.0	57.9	58.5	2161-2177 bp	1.0	81.4
ndhC-trnV-TAC_p5	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	2078-2094 bp	1.0	81.1