Pi
0.0394
MarkerSeek score
79.00
Primer available
yes
Label
trnT-TGT-trnL-TAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-TGT-trnL-TAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 534-574 bp | 1.0 | 86.9 |
| trnT-TGT-trnL-TAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 565-605 bp | 1.0 | 86.5 |
| trnT-TGT-trnL-TAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 542-582 bp | 1.0 | 86.3 |
| trnT-TGT-trnL-TAA_p4 | GAGTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 573-613 bp | 1.0 | 85.9 |
| trnT-TGT-trnL-TAA_p5 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 548-588 bp | 1.0 | 84.9 |