Hotspot

ycf3-trnS-GGA

LSC · 47298-48152 · 855 bp

Back to Cabomba

Pi 0.0346

MarkerSeek score 71.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 39
pi: 0.0346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGAGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	45.5	50.0	57.2	58.1	749-966 bp	1.0	64.3
ycf3-trnS-GGA_p2	CCATTTATCCGAGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	45.5	50.0	57.2	58.2	770-987 bp	1.0	63.8
ycf3-trnS-GGA_p3	CCATTTATCCGAGATCTAGGCA	TTCCAATGCTACGCCTTCAA	45.5	45.0	57.2	57.8	720-937 bp	1.0	63.6
ycf3-trnS-GGA_p4	CCATTTATCCGAGATCTAGGCA	TTTCCAATGCTACGCCTTCA	45.5	45.0	57.2	57.8	721-938 bp	1.0	63.6
ycf3-trnS-GGA_p5	TCCATTTATCCGAGATCTAGGC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	56.9	58.1	750-967 bp	1.0	62.2