Hotspot

psaA-ycf3

LSC · 44552-45233 · 682 bp

Back to Cabomba

Pi 0.0305

MarkerSeek score 71.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 28
pi: 0.0305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TCTTCACTTCTGGTTTCGGC	TTGGTTGAAGATCACAGGGC	50.0	50.0	58.1	58.1	734-747 bp	1.0	84.2
psaA-ycf3_p2	TCTTCACTTCTGGTTTCGGC	CACAGGGCGGTTCGAATAA	50.0	52.6	58.1	57.9	722-735 bp	1.0	75.9
psaA-ycf3_p3	TCTTCACTTCTGGTTTCGGC	ATCACAGGGCGGTTCGAATA	50.0	50.0	58.1	59.2	724-737 bp	1.0	75.7
psaA-ycf3_p4	TCTTCACTTCTGGTTTCGGC	GAAGATCACAGGGCGGTTC	50.0	57.9	58.1	58.2	728-741 bp	1.0	75.2
psaA-ycf3_p5	ACTTCTGGTTTCGGCGAAC	TTGGTTGAAGATCACAGGGC	52.6	50.0	58.4	58.1	729-742 bp	1.0	74.2