Hotspot

psbZ-trnG-GCC

LSC · 38872-39147 · 276 bp

Back to Cabomba

Pi 0.0428

MarkerSeek score 80.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 17
pi: 0.0428
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GTATTTGCTTCTCCCGAGGG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	438-449 bp	1.0	84.2
psbZ-trnG-GCC_p2	GTATTTGCTTCTCCCGAGGG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	439-450 bp	1.0	84.2
psbZ-trnG-GCC_p3	GTATTTGCTTCTCCCGAGGG	ACATTACTCCATCATGGGCG	55.0	50.0	58.0	57.7	467-478 bp	1.0	82.3
psbZ-trnG-GCC_p4	GTATTTGCTTCTCCCGAGGG	TACTCCATCATGGGCGGATA	55.0	50.0	58.0	57.7	463-474 bp	1.0	81.9
psbZ-trnG-GCC_p5	TATTTGCTTCTCCCGAGGGT	AATCGAACCCGCATCTTCTC	50.0	50.0	58.4	58.1	437-448 bp	1.0	81.2