Hotspot

trnE-TTC-trnT-GGT

LSC · 33230-33943 · 714 bp

Back to Cabomba

Pi 0.0341
MarkerSeek score 73.00
Primer available yes
Label trnE-TTC-trnT-GGT

Feature Metadata

label_name
trnE-TTC-trnT-GGT
feature_type
igs
region
LSC
length_bp
714
variable_sites
29
pi
0.0341
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-TTC-trnT-GGT_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 537-852 bp 1.0 86.0
trnE-TTC-trnT-GGT_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 535-850 bp 1.0 86.0
trnE-TTC-trnT-GGT_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 516-831 bp 1.0 85.6
trnE-TTC-trnT-GGT_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 514-829 bp 1.0 85.6
trnE-TTC-trnT-GGT_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 534-849 bp 1.0 85.5