Hotspot

petN-psbM

LSC · 30266-31557 · 1292 bp

Back to Cabomba

Pi 0.0301

MarkerSeek score 67.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 50
pi: 0.0301
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACGTTTTCCCTTTCACTCGT	ACGTAAAAACAGTAAGTCAAAACGA	45.0	32.0	57.7	57.9	1106-1422 bp	1.0	47.1
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACAGTAAGTCAAAACGA	50.0	32.0	57.6	57.9	1083-1399 bp	1.0	47.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTAAGTCAAAACGA	50.0	32.0	58.2	57.9	1144-1460 bp	1.0	47.0
petN-psbM_p4	GGGGGCATAATTCACATGGA	ACGTAAAAACAGTAAGTCAAAACGA	50.0	32.0	57.6	57.9	1166-1482 bp	1.0	46.9
petN-psbM_p5	AGGGGGCATAATTCACATGG	ACGTAAAAACAGTAAGTCAAAACGA	50.0	32.0	57.6	57.9	1167-1483 bp	1.0	46.9