Hotspot

trnS-GCT-trnG-TCC

LSC · 9187-10242 · 1056 bp

Back to Cabomba

Pi 0.0643

MarkerSeek score 79.90

Primer available yes

Label trnS-GCT-trnG-TCC

Feature Metadata

label_name: trnS-GCT-trnG-TCC
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 70
pi: 0.0643
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnG-TCC_p1	ACGGAGAAAGAGGGATTCGA	AACCGAAAGAACCCTTAACT	50.0	40.0	58.2	54.5	971-1217 bp	1.0	59.7
trnS-GCT-trnG-TCC_p2	ACGGAGAAAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	933-1179 bp	1.0	59.7
trnS-GCT-trnG-TCC_p3	GGGTACGGAGAAAGAGGGAT	AACCGAAAGAACCCTTAACT	55.0	40.0	58.2	54.5	975-1221 bp	1.0	59.2
trnS-GCT-trnG-TCC_p4	GGGTACGGAGAAAGAGGGAT	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	937-1183 bp	1.0	59.2
trnS-GCT-trnG-TCC_p5	AATCTGCCGCTTTAGTCCAC	AACCGAAAGAACCCTTAACT	50.0	40.0	58.3	54.5	916-1162 bp	1.0	59.1