Hotspot

trnK-TTT-rps16

LSC · 4301-5083 · 783 bp

Back to Cabomba

Pi 0.0423
MarkerSeek score 76.40
Primer available yes
Label trnK-TTT-rps16

Feature Metadata

label_name
trnK-TTT-rps16
feature_type
igs
region
LSC
length_bp
783
variable_sites
46
pi
0.0423
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-TTT-rps16_p1 CCTTTCAGGATCAGTCGTGG GGAAAAAGGAGCTCAACCCA 55.0 50.0 58.0 58.0 954-1000 bp 0.666667 73.0
trnK-TTT-rps16_p2 CCTTTCAGGATCAGTCGTGG TGGAAAAAGGAGCTCAACCC 55.0 50.0 58.0 58.0 955-1001 bp 0.666667 73.0
trnK-TTT-rps16_p3 TCAGGATCAGTCGTGGTCTT GGAAAAAGGAGCTCAACCCA 50.0 50.0 58.1 58.0 950-996 bp 0.666667 72.5
trnK-TTT-rps16_p4 TCAGGATCAGTCGTGGTCTT TGGAAAAAGGAGCTCAACCC 50.0 50.0 58.1 58.0 951-997 bp 0.666667 72.5
trnK-TTT-rps16_p5 TTCAGGATCAGTCGTGGTCT GGAAAAAGGAGCTCAACCCA 50.0 50.0 58.1 58.0 951-997 bp 0.666667 72.5