Hotspot

trnH-GTG-psbA

LSC · 77-309 · 233 bp

Back to Cabomba

Pi 0.069

MarkerSeek score 73.50

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 23
pi: 0.069
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	AATCCACTTGGCTACATCCG	GTTGACAGTCAAGGTCGTGT	50.0	50.0	57.7	58.1	397-398 bp	1.0	81.9
trnH-GTG-psbA_p2	AATCCACTTGGCTACATCCG	TGCATGGTTCCTTGGTAACC	50.0	50.0	57.7	58.1	675-676 bp	1.0	79.9
trnH-GTG-psbA_p3	AATCCACTTGGCTACATCCG	CGTATTCAGCTCCTGTTGCT	50.0	50.0	57.7	58.0	879-880 bp	1.0	79.8
trnH-GTG-psbA_p4	AATCCACTTGGCTACATCCG	CTGTTGCGTATTCAGCTCCT	50.0	50.0	57.7	58.0	885-886 bp	1.0	79.7
trnH-GTG-psbA_p5	AATCCACTTGGCTACATCCG	GTGAGTGGGAGCTTAGCTTC	50.0	55.0	57.7	58.0	930-931 bp	1.0	79.6