Hotspot

rbcL

LSC · 58403-59851 · 1449 bp

Back to Byrsonima

Pi 0.00483

MarkerSeek score 47.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 7
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TTTTCAAGCGCGGAAATTCC	GATACTTGGCTCGACCCATG	45.0	55.0	57.9	58.1	2515-2516 bp	1.0	78.8
rbcL_p2	GGGTTGCGCCATACATATGA	GATACTTGGCTCGACCCATG	50.0	55.0	58.1	58.1	1970 bp	1.0	78.4
rbcL_p3	CTTTGCGAAAGATTCCTGCG	GATACTTGGCTCGACCCATG	50.0	55.0	58.1	58.1	1862 bp	1.0	78.4
rbcL_p4	TTTTCAAGCGCGGAAATTCC	CGAAATGGAACGGAAGGGAA	45.0	50.0	57.9	58.2	2697 bp	1.0	78.3
rbcL_p5	TTTTCAAGCGCGGAAATTCC	TTCGAAATGGAACGGAAGGG	45.0	50.0	57.9	58.2	2699 bp	1.0	78.3