Hotspot

ndhD

SSC · 120515-122005 · 1491 bp

Back to Byrsonima

Pi 0.00134

MarkerSeek score 50.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1491
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGATTGCTTTTTGATTTTTGT	GTACGGGTTTTTCTGGTCCA	27.3	50.0	53.6	57.7	1588 bp	1.0	40.1
ndhD_p2	AGGATTGCTTTTTGATTTTTGT	ACGGGTTTTTCTGGTCCAAG	27.3	50.0	53.6	58.3	1586 bp	1.0	40.1
ndhD_p3	AGGATTGCTTTTTGATTTTTGT	CGGGTTTTTCTGGTCCAAGT	27.3	50.0	53.6	58.3	1585 bp	1.0	40.1
ndhD_p4	AGGATTGCTTTTTGATTTTTGT	TGTTTACCGAAAAAGCCCGT	27.3	45.0	53.6	58.3	1648 bp	1.0	40.1
ndhD_p5	AGGATTGCTTTTTGATTTTTGTT	GTACGGGTTTTTCTGGTCCA	26.1	50.0	54.4	57.7	1588 bp	1.0	40.1