Hotspot

atpA

LSC · 11192-12703 · 1512 bp

Back to Byrsonima

Pi 0.00198

MarkerSeek score 46.70

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	AGCCATTGAACAGCTGGAAA	50.0	45.0	58.0	58.0	2532-2556 bp	1.0	80.0
atpA_p2	AGCCTTCCAAGCTAACGATG	TGTTTGGGTCGATGCAAGAA	50.0	45.0	58.0	58.0	2227-2251 bp	1.0	79.8
atpA_p3	CCTAGCCTTCCAAGCTAACG	AGCCATTGAACAGCTGGAAA	55.0	45.0	58.1	58.0	2535-2559 bp	1.0	79.7
atpA_p4	CCTAGCCTTCCAAGCTAACG	TGTTTGGGTCGATGCAAGAA	55.0	45.0	58.1	58.0	2230-2254 bp	1.0	79.6
atpA_p5	AGCCTTCCAAGCTAACGATG	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.1	2670-2694 bp	1.0	79.2