Hotspot

ccsA-ndhD

SSC · 120277-120514 · 238 bp

Back to Byrsonima

Pi 0.042

MarkerSeek score 71.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 238
variable_sites: 10
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTCGCTTCTATTGGCTTTCT	CAGTTGACAAGGTCGAAGCT	42.9	50.0	57.7	58.1	382-387 bp	1.0	70.4
ccsA-ndhD_p2	TGTCGCTTCTATTGGCTTTCT	TCAGTTGACAAGGTCGAAGC	42.9	50.0	57.7	58.1	383-388 bp	1.0	70.3
ccsA-ndhD_p3	TCATTCTGCAATTGTCGCTTC	CAGTTGACAAGGTCGAAGCT	42.9	50.0	57.5	58.1	394-399 bp	1.0	69.2
ccsA-ndhD_p4	TCATTCTGCAATTGTCGCTTC	TCAGTTGACAAGGTCGAAGC	42.9	50.0	57.5	58.1	395-400 bp	1.0	69.1
ccsA-ndhD_p5	CATTCTGCAATTGTCGCTTCT	CAGTTGACAAGGTCGAAGCT	42.9	50.0	57.5	58.1	393-398 bp	1.0	69.1