Hotspot

psaI-ycf4

LSC · 63417-63886 · 470 bp

Back to Byrsonima

Pi 0.0107

MarkerSeek score 62.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 5
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GTAGGCTTAGTCTTTCCGGC	TCCAACCAAGAAAAACCCCA	55.0	45.0	58.1	57.4	657-659 bp	1.0	75.9
psaI-ycf4_p2	TTTCCGGCAATTGCAATGTC	TCCAACCAAGAAAAACCCCA	45.0	45.0	57.9	57.4	645-647 bp	1.0	75.8
psaI-ycf4_p3	CTTTCCGGCAATTGCAATGT	TCCAACCAAGAAAAACCCCA	45.0	45.0	57.9	57.4	646-648 bp	1.0	75.8
psaI-ycf4_p4	TAGTCTTTCCGGCAATTGCA	TCCAACCAAGAAAAACCCCA	45.0	45.0	57.8	57.4	650-652 bp	1.0	74.9
psaI-ycf4_p5	GTAGGCTTAGTCTTTCCGGC	TTGTTTTTCGAGACCCCACT	55.0	45.0	58.1	57.3	598-600 bp	1.0	74.7