Hotspot

psbM-trnD

LSC · 31686-32764 · 1079 bp

Back to Byrsonima

Pi 0.00188

MarkerSeek score 60.20

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	GGGACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	1196-1214 bp	1.0	66.0
psbM-trnD_p2	AGGGACTAGAATGAACAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.4	58.5	1197-1215 bp	1.0	63.8
psbM-trnD_p3	AGGCAGTAGGGACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.3	58.5	1204-1222 bp	1.0	63.2
psbM-trnD_p4	GGGACTAGAATGAACAGTGCA	GTTCAATTGGTCAGAGCACC	47.6	50.0	57.7	57.0	1197-1215 bp	1.0	61.9
psbM-trnD_p5	AGGCAGTAGGGACTAGAATGAA	TTCAATTGGTCAGAGCACCG	45.5	50.0	58.0	58.5	1204-1222 bp	1.0	60.7