Hotspot

trnS-trnG-GCC

LSC · 8957-9850 · 894 bp

Back to Byrsonima

Pi 0.00345
MarkerSeek score 60.70
Primer available yes
Label trnS-trnG-GCC

Feature Metadata

label_name
trnS-trnG-GCC
feature_type
igs
region
LSC
length_bp
894
variable_sites
3
pi
0.00345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GAATATGAGCCGAGGGATCC 50.0 55.0 58.0 57.3 1006-1027 bp 1.0 76.7
trnS-trnG-GCC_p2 CAATCCGACGCTTTAGTCCA GGAATATGAGCCGAGGGATC 50.0 55.0 58.0 57.3 1007-1028 bp 1.0 76.7
trnS-trnG-GCC_p3 AATCCGACGCTTTAGTCCAC GAATATGAGCCGAGGGATCC 50.0 55.0 58.0 57.3 1005-1026 bp 1.0 76.7
trnS-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC GGAATATGAGCCGAGGGATC 50.0 55.0 58.0 57.3 1006-1027 bp 1.0 76.7
trnS-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA AATATGAGCCGAGGGATCCC 50.0 55.0 58.0 58.7 1005-1026 bp 1.0 76.4