Hotspot

trnH-GUG-psbA

LSC · 188-463 · 276 bp

Back to Byrsonima

Pi 0.0145
MarkerSeek score 60.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
276
variable_sites
4
pi
0.0145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 TGGTTAGTTATGGGCGAACG CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.0 57.9 408 bp 1.0 80.2
trnH-GUG-psbA_p2 TGGTTAGTTATGGGCGAACG TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.0 57.9 411 bp 1.0 80.1
trnH-GUG-psbA_p3 TTAGTTATGGGCGAACGACG CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.1 57.9 405 bp 1.0 79.6
trnH-GUG-psbA_p4 TTAGTTATGGGCGAACGACG TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.1 57.9 408 bp 1.0 79.5
trnH-GUG-psbA_p5 TGGTTAGTTATGGGCGAACG TGGAAGTTATGCACGAACGT 50.0 45.0 58.0 57.8 443 bp 1.0 79.4