Hotspot

ndhI

SSC · 121497-122024 · 528 bp

Pi 0.0647

MarkerSeek score 82.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 528
variable_sites: 67
pi: 0.0647
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AAATAAGGAGTTGTGCGGCA	ATATGGTCTTTGGGGGTGGA	45.0	50.0	58.1	58.0	1693 bp	0.25	49.2
ndhI_p2	AAATAAGGAGTTGTGCGGCA	GGTCTTTGGGGGTGGAATTT	45.0	50.0	58.1	58.0	1689 bp	0.25	49.0
ndhI_p3	AAATAAGGAGTTGTGCGGCA	GAATTTGTGGCGTCAACCTG	45.0	50.0	58.1	57.9	1675 bp	0.25	48.8
ndhI_p4	AAATAAGGAGTTGTGCGGCA	GGAGCCATATGAGGTGCAAA	45.0	50.0	58.1	57.9	1816 bp	0.25	48.8
ndhI_p5	TATTGATGACCCGACCAGGA	ATATGGTCTTTGGGGGTGGA	50.0	50.0	57.8	58.0	1451 bp	0.25	48.6