Hotspot

accD

LSC · 59415-60950 · 1536 bp

Pi 0.0717

MarkerSeek score 82.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 208
pi: 0.0717
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCGTGATACTTCCGGTTCTT	TGCTCGGAGAAAACCTCATG	50.0	50.0	58.0	57.9	2195 bp	0.25	49.2
accD_p2	GCGTGATACTTCCGGTTCTT	GCTCGGAGAAAACCTCATGT	50.0	50.0	58.0	57.9	2194 bp	0.25	49.1
accD_p3	GCGTGATACTTCCGGTTCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2032 bp	0.25	49.1
accD_p4	GAGGCTAGCAAATGGAGTCC	TGCTCGGAGAAAACCTCATG	55.0	50.0	58.0	57.9	2698 bp	0.25	48.9
accD_p5	GAGGCTAGCAAATGGAGTCC	GCTCGGAGAAAACCTCATGT	55.0	50.0	58.0	57.9	2697 bp	0.25	48.9