Hotspot

trnN-GUU-trnR-ACG

IRa · 132355-132973 · 619 bp

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Pi 0.0502
MarkerSeek score 82.30
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
619
variable_sites
56
pi
0.0502
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC TGTCCCCTCCAGTCAAGAAT 55.0 50.0 58.0 58.0 1220-1223 bp 1.0 87.6
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC CCTTGTTCCTACATGACCCG 55.0 55.0 58.0 58.0 1370-1394 bp 1.0 87.3
trnN-GUU-trnR-ACG_p3 CCGTTGTTCCTCAGTAGCTC CAGCTGAGGCATCCTAACAG 55.0 55.0 58.0 58.0 1405-1429 bp 1.0 86.9
trnN-GUU-trnR-ACG_p4 CCGTTGTTCCTCAGTAGCTC GTCAAGTGGAAGTGCAGTGA 55.0 50.0 58.0 58.0 1431-1455 bp 1.0 86.8
trnN-GUU-trnR-ACG_p5 CCGTTGTTCCTCAGTAGCTC CAATTCGATCAGGCATTCGC 55.0 50.0 58.0 58.0 1348 bp 0.25 49.8