Hotspot

ndhF-rpl32

SSC · 114887-115829 · 943 bp

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Pi 0.379
MarkerSeek score 70.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
943
variable_sites
304
pi
0.379
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.37
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACCACACATGAAGGGGAAAC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 2389 bp 0.25 49.9
ndhF-rpl32_p2 ACCACACATGAAGGGGAAAC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 2390 bp 0.25 49.9
ndhF-rpl32_p3 ACACATCCCAACCAATTCCC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 2112 bp 0.25 49.4
ndhF-rpl32_p4 ACACATCCCAACCAATTCCC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 2113 bp 0.25 49.4
ndhF-rpl32_p5 CACATCCCAACCAATTCCCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 2111 bp 0.25 49.4