Hotspot

petN-psbM

LSC · 29441-30295 · 855 bp

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Pi 0.171
MarkerSeek score 83.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
855
variable_sites
271
pi
0.171
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTGAGTACTGGGGCATTCA TGTCATTCCCAAGGGATCCT 50.0 50.0 58.0 58.0 2015 bp 0.25 49.5
petN-psbM_p2 TCTGAGTACTGGGGCATTCA AGTCCCGACGGATCAAAATG 50.0 50.0 58.0 58.0 2121 bp 0.25 49.4
petN-psbM_p3 TCTGAGTACTGGGGCATTCA CAGTCCCGACGGATCAAAAT 50.0 50.0 58.0 58.0 2122 bp 0.25 49.4
petN-psbM_p4 ATACTTCCAAAAGCGTCGCT TGTCATTCCCAAGGGATCCT 45.0 50.0 57.9 58.0 1667 bp 0.25 49.1
petN-psbM_p5 ATACTTCCAAAAGCGTCGCT AGTCCCGACGGATCAAAATG 45.0 50.0 57.9 58.0 1773 bp 0.25 49.0