Hotspot

trnC-GCA-petN

LSC · 28444-29350 · 907 bp

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Pi 0.134
MarkerSeek score 84.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
907
variable_sites
202
pi
0.134
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.84
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 862-973 bp 1.0 90.1
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 923-1034 bp 1.0 88.5
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 880-991 bp 1.0 87.9
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 941-1052 bp 1.0 86.3
trnC-GCA-petN_p5 GGTGTCGCCTGATCAACAAA AGCCCAAGCGAGACTTACTA 50.0 50.0 58.8 58.2 843-954 bp 1.0 84.7