Hotspot

atpH-atpI

LSC · 13781-14970 · 1190 bp

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Pi 0.133
MarkerSeek score 82.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1190
variable_sites
280
pi
0.133
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.89
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ACAGAAGCGGCAGAAATCAG CCATGGAGGGTCATCATTGA 50.0 50.0 58.6 57.0 1171-1239 bp 1.0 79.3
atpH-atpI_p2 AGAAGCGGCAGAAATCAGTG CCATGGAGGGTCATCATTGA 50.0 50.0 58.6 57.0 1169-1237 bp 1.0 79.3
atpH-atpI_p3 AGAAGCGGCAGAAATCAGTG TCCATGGAGGGTCATCATTG 50.0 50.0 58.6 57.0 1170-1238 bp 1.0 79.3
atpH-atpI_p4 CAGAAGCGGCAGAAATCAGT CCATGGAGGGTCATCATTGA 50.0 50.0 58.6 57.0 1170-1238 bp 1.0 79.3
atpH-atpI_p5 CAGAAGCGGCAGAAATCAGT TCCATGGAGGGTCATCATTG 50.0 50.0 58.6 57.0 1171-1239 bp 1.0 79.3