Hotspot

trnK-UUU-rps16

LSC · 4367-4954 · 588 bp

Back to Buxus

Pi 0.155
MarkerSeek score 83.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
588
variable_sites
170
pi
0.155
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GCATTTTGTTTTGCGCCAAG TTAGAACCCCCAACCCTCAT 45.0 50.0 58.0 57.9 1409 bp 0.25 49.3
trnK-UUU-rps16_p2 GCATTTTGTTTTGCGCCAAG CGATCGCGAAGAGAAGGAAA 45.0 50.0 58.0 58.1 1052 bp 0.25 49.1
trnK-UUU-rps16_p3 CCTTCATCTCCACCCCCTAA TTAGAACCCCCAACCCTCAT 55.0 50.0 58.1 57.9 1655 bp 0.25 48.6
trnK-UUU-rps16_p4 GCATTTTGTTTTGCGCCAAG TTTAGAACCCCCAACCCTCA 45.0 50.0 58.0 58.2 1410 bp 0.25 48.5
trnK-UUU-rps16_p5 CCTTCATCTCCACCCCCTAA CGATCGCGAAGAGAAGGAAA 55.0 50.0 58.1 58.1 1298 bp 0.25 48.4