primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	CATCTTTCACCCAGCATCGA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.196840	3601	4329	1267-1267	0.250000	48.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	CATCTTTCACCCAGCATCGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.196840	3601	4329	1266-1266	0.250000	48.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	CATCTTTCACCCAGCATCGA	GGGAGGGTTGCTAACTCAAC	20	20	50.0	55.0	58.0	58.2	0.0	2.5	0.205914	3601	4329	1284-1284	0.250000	48.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCCAGCATCGAAGGATTTGA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.211106	3601	4329	1258-1258	0.250000	48.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCCAGCATCGAAGGATTTGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.211106	3601	4329	1257-1257	0.250000	48.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GCATTTTGTTTTGCGCCAAG	TTAGAACCCCCAACCCTCAT	20	20	45.0	50.0	58.0	57.9	4.2	0.0	0.090274	4367	4954	1409-1409	0.250000	49.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GCATTTTGTTTTGCGCCAAG	CGATCGCGAAGAGAAGGAAA	20	20	45.0	50.0	58.0	58.1	4.2	15.2	0.112067	4367	4954	1052-1052	0.250000	49.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTCATCTCCACCCCCTAA	TTAGAACCCCCAACCCTCAT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.175516	4367	4954	1655-1655	0.250000	48.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GCATTTTGTTTTGCGCCAAG	TTTAGAACCCCCAACCCTCA	20	20	45.0	50.0	58.0	58.2	4.2	0.0	0.185547	4367	4954	1410-1410	0.250000	48.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTCATCTCCACCCCCTAA	CGATCGCGAAGAGAAGGAAA	20	20	55.0	50.0	58.1	58.1	0.0	15.2	0.197309	4367	4954	1298-1298	0.250000	48.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TTTCCTTCTCTTCGCGATCG	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	58.1	58.0	10.9	1.2	0.118911	5255	6270	1728-1728	0.250000	49.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTTCCTTCTCTTCGCGATCG	TCGAAAACTGACAGCAGCTT	20	20	50.0	45.0	58.1	58.0	10.9	1.2	0.137065	5255	6270	1729-1729	0.250000	48.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TTTCCTTCTCTTCGCGATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	10.9	16.9	0.166725	5255	6270	1132-1132	0.250000	48.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGTTTTCCTTCTCTTCGCGA	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	58.2	58.0	3.7	1.2	0.236281	5255	6270	1731-1731	0.250000	48.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGTTTTCCTTCTCTTCGCGA	TCGAAAACTGACAGCAGCTT	20	20	50.0	45.0	58.2	58.0	3.7	1.2	0.254435	5255	6270	1732-1732	0.250000	48.0
atpH-atpI_p1	atpH-atpI	1	ACAGAAGCGGCAGAAATCAG	CCATGGAGGGTCATCATTGA	20	20	50.0	50.0	58.6	57.0	1.4	5.6	1.589069	13781	14970	1171-1239	1.000000	79.3
atpH-atpI_p2	atpH-atpI	2	AGAAGCGGCAGAAATCAGTG	CCATGGAGGGTCATCATTGA	20	20	50.0	50.0	58.6	57.0	1.4	5.6	1.589069	13781	14970	1169-1237	1.000000	79.3
atpH-atpI_p3	atpH-atpI	3	AGAAGCGGCAGAAATCAGTG	TCCATGGAGGGTCATCATTG	20	20	50.0	50.0	58.6	57.0	1.4	11.4	1.589069	13781	14970	1170-1238	1.000000	79.3
atpH-atpI_p4	atpH-atpI	4	CAGAAGCGGCAGAAATCAGT	CCATGGAGGGTCATCATTGA	20	20	50.0	50.0	58.6	57.0	1.4	5.6	1.589069	13781	14970	1170-1238	1.000000	79.3
atpH-atpI_p5	atpH-atpI	5	CAGAAGCGGCAGAAATCAGT	TCCATGGAGGGTCATCATTG	20	20	50.0	50.0	58.6	57.0	1.4	11.4	1.589069	13781	14970	1171-1239	1.000000	79.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	GCTTTTGTTGATCAGGCGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058025	27356	28362	1603-1680	1.000000	91.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AGAGGTTCCCAGGGAATTCA	GCTTTTGTTGATCAGGCGAC	20	20	50.0	50.0	57.9	58.0	24.7	0.0	0.090653	27356	28362	1430-1507	1.000000	91.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CGAGCCCATATCCTTGCTTT	TGAATGCCCCAGTACTCAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075919	27356	28362	1954-1954	0.250000	49.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCGTTATGGTCCGCTTC	GCTTTTGTTGATCAGGCGAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081431	27356	28362	1519-1519	0.250000	49.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CGAGCCCATATCCTTGCTTT	GACAAAGTACCTTACGCCGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.097390	27356	28362	1916-1916	0.250000	49.2
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28444	29350	862-973	1.000000	90.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28444	29350	923-1034	1.000000	88.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28444	29350	880-991	1.000000	87.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	28444	29350	941-1052	1.000000	86.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGTGTCGCCTGATCAACAAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.8	58.2	0.0	0.0	0.916527	28444	29350	843-954	1.000000	84.7
petN-psbM_p1	petN-psbM	1	TCTGAGTACTGGGGCATTCA	TGTCATTCCCAAGGGATCCT	20	20	50.0	50.0	58.0	58.0	0.0	12.6	0.063169	29441	30295	2015-2015	0.250000	49.5
petN-psbM_p2	petN-psbM	2	TCTGAGTACTGGGGCATTCA	AGTCCCGACGGATCAAAATG	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.072966	29441	30295	2121-2121	0.250000	49.4
petN-psbM_p3	petN-psbM	3	TCTGAGTACTGGGGCATTCA	CAGTCCCGACGGATCAAAAT	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.072966	29441	30295	2122-2122	0.250000	49.4
petN-psbM_p4	petN-psbM	4	ATACTTCCAAAAGCGTCGCT	TGTCATTCCCAAGGGATCCT	20	20	45.0	50.0	57.9	58.0	15.5	12.6	0.109141	29441	30295	1667-1667	0.250000	49.1
petN-psbM_p5	petN-psbM	5	ATACTTCCAAAAGCGTCGCT	AGTCCCGACGGATCAAAATG	20	20	45.0	50.0	57.9	58.0	15.5	17.5	0.118938	29441	30295	1773-1773	0.250000	49.0
trnL-UAA_p1	trnL-UAA	1	GGAAGGTCTGCTCTAGGGAA	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	58.1	58.0	0.0	17.4	0.135142	49315	49936	1517-1517	0.250000	48.9
trnL-UAA_p2	trnL-UAA	2	ATGGGTCGTCGAGAAAGAGA	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	58.2	58.0	0.0	17.4	0.167011	49315	49936	1253-1253	0.250000	48.7
trnL-UAA_p3	trnL-UAA	3	GGAAGGTCTGCTCTAGGGAA	CGTAGAGCTCCAGACACCTA	20	20	55.0	55.0	58.1	58.0	0.0	10.9	0.172455	49315	49936	1683-1683	0.250000	48.6
trnL-UAA_p4	trnL-UAA	4	TCATTCGGAAAGGTGGAAGC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	58.2	58.0	0.0	17.4	0.181289	49315	49936	1425-1425	0.250000	48.5
trnL-UAA_p5	trnL-UAA	5	GGAAGGTCTGCTCTAGGGAA	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	58.1	58.1	0.0	20.1	0.184533	49315	49936	1553-1553	0.250000	48.5
accD_p1	accD	1	GCGTGATACTTCCGGTTCTT	TGCTCGGAGAAAACCTCATG	20	20	50.0	50.0	58.0	57.9	0.0	9.7	0.105112	59415	60950	2195-2195	0.250000	49.2
accD_p2	accD	2	GCGTGATACTTCCGGTTCTT	GCTCGGAGAAAACCTCATGT	20	20	50.0	50.0	58.0	57.9	0.0	9.7	0.106575	59415	60950	2194-2194	0.250000	49.1
accD_p3	accD	3	GCGTGATACTTCCGGTTCTT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.109508	59415	60950	2032-2032	0.250000	49.1
accD_p4	accD	4	GAGGCTAGCAAATGGAGTCC	TGCTCGGAGAAAACCTCATG	20	20	55.0	50.0	58.0	57.9	18.2	9.7	0.138725	59415	60950	2698-2698	0.250000	48.9
accD_p5	accD	5	GAGGCTAGCAAATGGAGTCC	GCTCGGAGAAAACCTCATGT	20	20	55.0	50.0	58.0	57.9	18.2	9.7	0.140188	59415	60950	2697-2697	0.250000	48.9
rpl20-rps12_p1	rpl20-rps12	1	GACTGCTAGAACTGCTGGTC	TATGTCAGCAACAGAAGCCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117057	70514	71288	1806-1806	0.250000	49.1
rpl20-rps12_p2	rpl20-rps12	2	GACTGCTAGAACTGCTGGTC	CCGAGGAACATGTATTCGGG	20	20	55.0	55.0	58.0	58.1	0.0	25.9	0.137332	70514	71288	1458-1458	0.250000	48.9
rpl20-rps12_p3	rpl20-rps12	3	GACTGCTAGAACTGCTGGTC	TTGTTGATCGTGTAGCGGTT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.161031	70514	71288	1774-1774	0.250000	48.7
rpl20-rps12_p4	rpl20-rps12	4	GACTGCTAGAACTGCTGGTC	GGCAAACCCTTATGGATGGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.162376	70514	71288	1853-1853	0.250000	48.7
rpl20-rps12_p5	rpl20-rps12	5	GACTGCTAGAACTGCTGGTC	ATTTGTCCTGGAAGCGGAAG	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.176811	70514	71288	1923-1923	0.250000	48.6
ndhF-rpl32_p1	ndhF-rpl32	1	ACCACACATGAAGGGGAAAC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	16.3	0.0	0.012454	114887	115829	2389-2389	0.250000	49.9
ndhF-rpl32_p2	ndhF-rpl32	2	ACCACACATGAAGGGGAAAC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	16.3	0.0	0.013825	114887	115829	2390-2390	0.250000	49.9
ndhF-rpl32_p3	ndhF-rpl32	3	ACACATCCCAACCAATTCCC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070639	114887	115829	2112-2112	0.250000	49.4
ndhF-rpl32_p4	ndhF-rpl32	4	ACACATCCCAACCAATTCCC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072010	114887	115829	2113-2113	0.250000	49.4
ndhF-rpl32_p5	ndhF-rpl32	5	CACATCCCAACCAATTCCCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072031	114887	115829	2111-2111	0.250000	49.4
ndhI_p1	ndhI	1	AAATAAGGAGTTGTGCGGCA	ATATGGTCTTTGGGGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.101314	121497	122024	1693-1693	0.250000	49.2
ndhI_p2	ndhI	2	AAATAAGGAGTTGTGCGGCA	GGTCTTTGGGGGTGGAATTT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.119889	121497	122024	1689-1689	0.250000	49.0
ndhI_p3	ndhI	3	AAATAAGGAGTTGTGCGGCA	GAATTTGTGGCGTCAACCTG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.146190	121497	122024	1675-1675	0.250000	48.8
ndhI_p4	ndhI	4	AAATAAGGAGTTGTGCGGCA	GGAGCCATATGAGGTGCAAA	20	20	45.0	50.0	58.1	57.9	0.0	3.7	0.152851	121497	122024	1816-1816	0.250000	48.8
ndhI_p5	ndhI	5	TATTGATGACCCGACCAGGA	ATATGGTCTTTGGGGGTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.174060	121497	122024	1451-1451	0.250000	48.6
ycf1_p1	ycf1	1	GGTTTGTGTAGTCGGTCCAA	ACATACCTGCCGAGTTTGTC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.338364	130287	130406	968-968	0.250000	47.3
ycf1_p2	ycf1	2	GGTTTGTGTAGTCGGTCCAA	CCCTCACGCAGAAAGATCAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.342743	130287	130406	698-698	0.250000	47.3
ycf1_p3	ycf1	3	AAAGGGGTTTGTGTAGTCGG	ACATACCTGCCGAGTTTGTC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.360351	130287	130406	973-973	0.250000	47.1
ycf1_p4	ycf1	4	AAAGGGGTTTGTGTAGTCGG	CCCTCACGCAGAAAGATCAA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.364730	130287	130406	703-703	0.250000	47.1
ycf1_p5	ycf1	5	GGACGCATGGAGCCTTTTAT	ACATACCTGCCGAGTTTGTC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.414424	130287	130406	887-887	0.250000	46.7
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	TGTCCCCTCCAGTCAAGAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047124	132355	132973	1220-1223	1.000000	87.6
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026507	132355	132973	1370-1394	1.000000	87.3
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	20	20	55.0	55.0	58.0	58.0	0.0	8.4	0.051503	132355	132973	1405-1429	1.000000	86.9
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCGTTGTTCCTCAGTAGCTC	GTCAAGTGGAAGTGCAGTGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048772	132355	132973	1431-1455	1.000000	86.8
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CCGTTGTTCCTCAGTAGCTC	CAATTCGATCAGGCATTCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.030618	132355	132973	1348-1348	0.250000	49.8