Hotspot

rpl16

LSC · 80917-82467 · 1551 bp

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Pi 0.00396
MarkerSeek score 63.20
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1551
variable_sites
6
pi
0.00396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCAATGCAAAGGATGAAGGA AGGTTGAATAAAAATTCGATTGACCA 40.0 30.8 54.9 58.1 1662-1698 bp 1.0 42.0
rpl16_p2 TCAATGCAAAGGATGAAGGA GGTTGAATAAAAATTCGATTGACCA 40.0 32.0 54.9 56.9 1661-1697 bp 1.0 42.0
rpl16_p3 TCAATGCAAAGGATGAAGGAA AGGTTGAATAAAAATTCGATTGACCA 38.1 30.8 55.7 58.1 1662-1698 bp 1.0 42.0
rpl16_p4 TTCAATGCAAAGGATGAAGGA AGGTTGAATAAAAATTCGATTGACCA 38.1 30.8 55.7 58.1 1663-1699 bp 1.0 42.0
rpl16_p5 TCAATGCAAAGGATGAAGGAA GGTTGAATAAAAATTCGATTGACCA 38.1 32.0 55.7 56.9 1661-1697 bp 1.0 42.0