Hotspot

rps12-trnV-GAC

IRb · 98786-100332 · 1547 bp

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Pi 0.000665
MarkerSeek score 61.70
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1547
variable_sites
1
pi
0.000665
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA CGGGGGAGCAAGTCAAAATA 45.0 50.0 57.7 57.9 1661-1704 bp 1.0 79.0
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1600-1643 bp 1.0 78.8
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1599-1642 bp 1.0 78.8
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1604-1647 bp 1.0 78.7
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1602-1645 bp 1.0 77.8