Hotspot

petA-psbJ

LSC · 62217-63151 · 935 bp

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Pi 0.015
MarkerSeek score 67.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
935
variable_sites
14
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 58.0 58.3 1086-1087 bp 1.0 78.3
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 58.0 57.6 1082-1083 bp 1.0 77.4
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1069-1070 bp 1.0 77.4
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 58.0 57.6 1085-1086 bp 1.0 77.4
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT GGTTCATATTCCGGGTTGGG 50.0 55.0 58.2 58.3 1083-1084 bp 1.0 77.2