Hotspot

accD-psaI

LSC · 58172-59105 · 934 bp

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Pi 0.00432
MarkerSeek score 62.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
934
variable_sites
4
pi
0.00432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1044-1075 bp 1.0 80.5
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1045-1076 bp 1.0 80.5
accD-psaI_p3 CCACGGTTTCTTTCCCTTGA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 1050-1081 bp 1.0 79.9
accD-psaI_p4 TCCACGGTTTCTTTCCCTTG AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 1051-1082 bp 1.0 79.9
accD-psaI_p5 TCAGCTCCACGGTTTCTTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 1050-1081 bp 1.0 79.7