Hotspot

atpB-rbcL

LSC · 53669-54439 · 771 bp

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Pi 0.00259
MarkerSeek score 60.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
771
variable_sites
2
pi
0.00259
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGGGAAAAACGACATCCAGT GGTGACATAAGTCCCTCCCT 50.0 55.0 58.1 58.1 909 bp 1.0 78.5
atpB-rbcL_p2 TGGGCCAATAATTTGAGCGA GGTGACATAAGTCCCTCCCT 45.0 55.0 57.9 58.1 887 bp 1.0 78.1
atpB-rbcL_p3 GGGGAAAAACGACATCCAGT TGGTGACATAAGTCCCTCCC 50.0 55.0 58.1 58.4 910 bp 1.0 76.1
atpB-rbcL_p4 TGGGCCAATAATTTGAGCGA TGGTGACATAAGTCCCTCCC 45.0 55.0 57.9 58.4 888 bp 1.0 75.7
atpB-rbcL_p5 CTGGGCCAATAATTTGAGCG GGTGACATAAGTCCCTCCCT 50.0 55.0 57.5 58.1 888 bp 1.0 75.3