Hotspot

ndhC-trnV-UAC

LSC · 49279-50691 · 1413 bp

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Pi 0.00354
MarkerSeek score 60.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1413
variable_sites
5
pi
0.00354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAACCGGTGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1557-1565 bp 1.0 78.1
ndhC-trnV-UAC_p2 TAACCGGTGCTAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1549-1557 bp 1.0 78.0
ndhC-trnV-UAC_p3 TCCTTCACTAACCGGTGCTA AGCCCAATGCAACAAAATGC 50.0 45.0 58.1 58.5 1639-1647 bp 1.0 75.9
ndhC-trnV-UAC_p4 TAACCGGTGCTAAAACTCCG AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1631-1639 bp 1.0 75.8
ndhC-trnV-UAC_p5 TCCTTCACTAACCGGTGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 1556-1564 bp 1.0 75.3